Physical mapping of the KCNJ8 gene to bovine chromosome 5q3.2-q3.4.

Source/description: Porcine chromosome 8 is homologous to human chromosome 4, but a significant number of rearrangements in gene order exist when compared with human. Genes expected to linkage map specifically in quantitative trait loci (QTL) regions for uterine capacity and ovulation rate were chosen by their position on human chromosome 4 (UCSC version hg16 based on NCBI Build 34; Golden Path web site; http://genome.ucsc.edu/) and availability of EST sequences in TIGR Gene Indices (TIGR web site; http://www.tigr.org/tdb/ ssgi/) that aligned to human genes. Primer pairs (Table 1) were designed using primer 3 (code available at: http://www-geno me.wi.mit.edu/genome_software/other/primer3.html) to flank introns or 3¢-UTR regions of porcine genes for amplification and SNP discovery.